P-27: The Effect of Oral Morphine Consumptionon Plasma Corticosteron Density and PlacentaDevelopment in Pregnant Wistar Rats

Background: Previous studies have shown that morphine consumption during pregnancy may delay embryo development or cause abnormal nervous system function .The most attention of research focuses on the embryo, whereas it was not attended to the placenta as an important organ which is affected by opioides. The present study focused on the effect of maternal morphine consumption on development of maternal portion and fetal portion of placental in Wistar rats.Materials and Methods: In this research, we used 24 Wistar rats with 170-200g weight. The experimental groups after pregnancy received 0/05mg/ml of morphine by tap water while the control group received water. Treatment and control groups were anesthetized by chloroform on 10th and 14th day of pregnancy, placenta with uterus were removed surgically and fixed in 10% formaldehyde for 20 days. 1cc blood was received from pregnant mother, the density of blood corticosteron was determined by ELISA method after centrifugation .The fixed embryos underwent tissue processing, sectioning and staining with hematoxylin and eosin(H&E). Placenta was studied in the light of the thickness of layer, area of blood lacuna , number of maternal and fetal portion cells and thickness of endometrium by light microscope, SPSS analyses and Motic software. Results: Our studies indicated that plasma corticosteron in the treatment group showed severe in erease than the control group .Similar result repeated for the experimental group showing the thickness of maternal portions and fetal portions of placenta in day 10 -14 of pregnancy, show the significant difference in experimental group (p≤0.05). Moreover, the increase number of both maternal and fetal portion cells of placenta and the decrease of blood lacuna area in both fetal and maternal portion Placenta were found, in the treatment group. Conclusion: In this study, the effects of morphine in increase the density of blood plasma corticoestron in addictive pregnant mothers were seen. also, all development of placenta in the experimental group with was delayed related to development in the control group. This may cause embryo abortion and defect of secretory function in placental hormones such as strogen and progestron which in turn may induce defect in infants development born from addictive mothers.